Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
![Alkali Scientific MES Monohydrate [2 (N Morpholino) ethanesulfonic acid], 1 Kg](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000661641.jpg-250.jpg)
Alkali Scientific MES Monohydrate [2 (N Morpholino) ethanesulfonic acid], 1 Kg
MES Monohydrate (2-(N-Morpholino) ethanesulfonic acid) is a biochemical reagent used for pH buffering in biochemical applications. This 1 kg volume is ideal for laboratories requiring large quantities of MES buffer for a variety of experiments. MES is commonly used in biochemical research, especially in enzyme assays, cell culture, and protein purification. It maintains a stable pH range, crucial for experiments that need precise and consistent conditions. The MES buffer ensures minimal interference in reactions, making it ideal for applications such as electrophoresis and chromatography. MES Monohydrate is a reliable and effective reagent used in a wide range of scientific studies, particularly in molecular biology and biochemistry.
![Alkali Scientific PBS [Phosphate Buffered Saline], 10X Powder Concentrate, White Granular Powder, 980g make, 10 L](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000661556.jpg-250.jpg)
Alkali Scientific PBS [Phosphate Buffered Saline], 10X Powder Concentrate, White Granular Powder, 980g make, 10 L
PBS [Phosphate Buffered Saline] 10X Powder Concentrate, in a 980g pack, makes 10 liters of 10X PBS solution. This highly concentrated powder is perfect for laboratories that require large volumes of PBS for a wide range of biological and biochemical applications. The powder dissolves easily in water, providing a quick and convenient way to prepare the solution. 10X PBS is essential in many laboratory protocols, including protein assays, cell culture, and washing procedures. The larger pack size is ideal for high-throughput laboratories or research facilities that need to prepare PBS solutions in bulk for routine use in experiments and testing.
eMolecules MES Monohydrate | 145224-94-8 | MFCD00149409 | 100g
Chem-Impex | MES Monohydrate | 100g | 288406858 | 21885 | | 145224-94-8 | MFCD00149409 | 213.250 | C6H15NO5S
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![Alkali Scientific MOPS [3 (N morpholino) propanesulfonic acid], 10 Kg](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000661636.jpg-250.jpg)
Alkali Scientific MOPS [3 (N morpholino) propanesulfonic acid], 10 Kg
MOPS (3-(N-morpholino)propane-1-sulfonic acid) is a biochemical reagent used for pH buffering in a variety of laboratory applications. This 10kg package of MOPS is ideal for large-scale production of buffer solutions, making it suitable for high-volume research and industrial applications. MOPS buffers are particularly effective in maintaining a stable pH range of 6.5 to 7.9, which is critical for biological assays, enzyme reactions, and protein analysis. It is commonly used in molecular biology, electrophoresis, and chromatography to stabilize proteins, nucleic acids, and enzymes. This bulk packaging ensures a cost-effective solution for labs with high buffer consumption.
Alkali Scientific TRIS Buffered Saline, 10X Solution, 4 L
TRIS Buffered Saline (TBS) 10X solution is a concentrated buffer commonly used in molecular biology, immunology, and biochemistry. This 4L bottle provides a convenient, large-volume supply of buffer for high-throughput laboratories and research facilities. TBS is widely used in protocols such as washing cells, diluting proteins, and preparing buffers for antibody conjugation in applications like western blotting and ELISA. The TRIS component ensures that the pH remains stable, preventing fluctuations that could affect the accuracy of experimental results. This 10X solution is easily diluted to 1X, making it ideal for a variety of laboratory applications where stable pH and ionic strength are essential.
Alkali Scientific Murashige & Skoog Medium with MES Buffer, 4.8 Grams of Powder, Makes 1 Liter of Solution
Murashige & Skoog Medium with MES Buffer is a specialized medium designed for plant tissue culture applications that require a stable pH environment. The 1-liter solution, made from 4.8 grams of powder, includes MES buffer, which helps stabilize the pH of the culture medium during growth. This formulation is critical in preventing pH shifts that could negatively affect plant cell growth. The MES buffer is ideal for studies in plant biotechnology, regeneration, and cell culture, where pH stability is essential for proper cellular functions. This medium is commonly used in laboratories focusing on plant tissue culture and genetic research, ensuring consistency in experimental conditions.
Cayman Chemical TrIs Buffer ConcentrAT10X 10mL
A thioredoxin inhibitor inhibits Trx activity in ES-2 cells at 20 UM cytotoxic to ES-2 cells (IC50 1.6 UM) induces ferroptosis in ES-2 cells at 5 UM an effect that can be blocked by DFO Trolox or ferrostatin-1 decreases tumor volume in a 4T1 murine mammary carcinoma model using immunocompetent but not immunodeficient mice at 50 mg/kg twice per week
Teknova DPBS, 500mL, DNase/RNase/Protease/Endotoxin Tested, Sterile. 8 Pack.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
Teknova 100mM Tris-HCl, pH 8.2. 1000mL, Sterile 6 pack.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Teknova 50mM Tris HCl, pH 7.5, 1000mL, DNase, RNase, Endotoxin Tested. Sterile. 8 pack.
50mM Tris HCl, pH 7.5, 1000mL, DNase, RNase, Endotoxin Tested. Sterile. 8 pack.
Teknova 0.45% Sterile Saline, 6mL, 25-Pack, Sterile. 12 Pack.
Sodium chloride is an essential ingredient in the lab. Not only does it help regulate the pH of solutions, but it also prevents growth of bacteria and other contaminants. In addition, salt is a powerful desiccant, meaning that it can help to dry out wet samples. As a result, salt is often used in a wide variety of laboratory procedures.
Alkali Scientific MES, Anhydrous, 1 Kg
MES, Anhydrous, 1 Kg, is a highly effective buffering reagent used in laboratories to maintain stable pH levels, particularly in biological, biochemical, and chemical applications. The anhydrous form of MES ensures that the buffer can be easily dissolved in water or other solvents for customized concentrations. It is primarily used in electrophoresis, enzyme reactions, and in preparing cell culture media. MES provides excellent buffering capacity in the pH range of 5.5 to 6.7, making it ideal for a variety of experiments that require precise pH control. The 1 kg weight is perfect for medium-scale laboratory use, offering both convenience and consistency in experiments.
![eMolecules 23141-25-5 | STRICTOSAMIDE | AstaTech | MFCD14105616 | 498.532 | C26H30N2O8 | 95.000 | OC[C@H]1O[C@@H](O[C@@H]2OC=C3[C@@H](C[C@@H]4N(CCc5c4[nH]c4ccccc54)C3=O)[C@H]2C=C)[C@H](O)[C@@H](O)[C@@H]1O | 1mg | 718087441](https://assets.fishersci.com/TFS-Assets/CCG/product-images/502460896.JPG-250.jpg)
eMolecules 23141-25-5 | STRICTOSAMIDE | AstaTech | MFCD14105616 | 498.532 | C26H30N2O8 | 95.000 | OC[C@H]1O[C@@H](O[C@@H]2OC=C3[C@@H](C[C@@H]4N(CCc5c4[nH]c4ccccc54)C3=O)[C@H]2C=C)[C@H](O)[C@@H](O)[C@@H]1O | 1mg | 718087441
STRICTOSAMIDE | AstaTech | 23141-25-5 | MFCD14105616 | 498.532 | C26H30N2O8 | 95.000 | OC[C@H]1O[C@@H](O[C@@H]2OC=C3[C@@H](C[C@@H]4N(CCc5c4[nH]c4ccccc54)C3=O)[C@H]2C=C)[C@H](O)[C@@H](O)[C@@H]1O | 1mg | 718087441
Teknova 1M Tris-HCl, pH 7.5. 500mL, Sterile, DNAse/RNAse Tested 8 pack.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
