Life Science Buffers
Apexbio Technology LLC Annexin V-Cy5/PI Apoptosis Kit 20assays
Annexin V is a Ca2 -dependent phospholipid-binding protein that specifically binds to phosphatidylserine (PS) Under normal conditions PS resides on the inner side of the cell membrane lipid bilayer However in early apoptosis PS translocates from the inner leaflet of the plasma membrane to the outer surface becoming exposed externally Annexin V exhibits high binding affinity for PS and when conjugated with fluorescent molecules (Cy5) it serves as a fluorescent probe to detect apoptosis using flow cytometry or fluorescence microscopy The staining procedure requires only 10 20 minutes PI specifically binds to double-stranded DNA to produce red fluorescence it is normally impermeant to intact cell membranes However during late-stage apoptosis or in dead cells PI penetrates the membrane to stain nuclear DNA Combined Annexin V-Cy5 and PI staining allows distinction between cells at various stages of apoptosis and dead cells
Miltenyi Biotec, Inc. CliniMACS PBS/EDTA Buffer 3 x 1L, HUD
Suitable for cell processing and cell separation in combination with the CliniMACS System.
This product is subject to the following: Miltenyi Biotec, Inc. Terms and Conditions of Sale For Products and Services
![Alkali Scientific DPBS 1X Solution [Dulbecco's Phosphate Buffered Saline 1X Sterile Solution], 500 Milliliters](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000661850.jpg-250.jpg)
Alkali Scientific DPBS 1X Solution [Dulbecco's Phosphate Buffered Saline 1X Sterile Solution], 500 Milliliters
DPBS 1X (Dulbecco's Phosphate Buffered Saline) is a sterile saline solution used in biological research and clinical laboratories. The 500mL bottle is a convenient size for smaller laboratories or specific experiments that require moderate volumes of buffer solution. DPBS is widely used for washing cells, preparing tissues, and diluting reagents in cell culture, molecular biology, and microbiology applications. The balanced composition of salts and phosphate ensures that the solution maintains osmotic pressure and pH stability, making it essential for maintaining cell viability and supporting biological assays that require precise environmental conditions.
Biotium Fixation Permeabilization Other Buffers Buffers Accessories and Background Reducers Fixation Permeabilization and Other Buffers Chemical Reagents 50/PK
PBS (phosphate-buffered saline) buffer powder packet for preparing 2 L of 1X PBS buffer Box includes 50 packets
![Alkali Scientific PBS [Phosphate Buffered Saline], 10X Powder Concentrate, White Granular Powder, 980g make, 10 L](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000661556.jpg-250.jpg)
Alkali Scientific PBS [Phosphate Buffered Saline], 10X Powder Concentrate, White Granular Powder, 980g make, 10 L
PBS [Phosphate Buffered Saline] 10X Powder Concentrate, in a 980g pack, makes 10 liters of 10X PBS solution. This highly concentrated powder is perfect for laboratories that require large volumes of PBS for a wide range of biological and biochemical applications. The powder dissolves easily in water, providing a quick and convenient way to prepare the solution. 10X PBS is essential in many laboratory protocols, including protein assays, cell culture, and washing procedures. The larger pack size is ideal for high-throughput laboratories or research facilities that need to prepare PBS solutions in bulk for routine use in experiments and testing.
Apexbio Technology LLC Annexin V-HF488/PI Apoptosis Kit 20assays
Annexin V is a Ca2 -dependent phospholipid-binding protein that specifically binds to phosphatidylserine (PS) Normally PS is localized on the inner leaflet of the cell membrane lipid bilayer During the early stages of apoptosis PS translocates from the inner leaflet to the outer surface of the cell membrane exposing it to the extracellular environment Due to Annexin V s high affinity for PS fluorescently labeled Annexin V (HF488) can be used as a fluorescent probe to detect apoptotic cells through flow cytometry or fluorescence microscopy with staining taking only 10-20 minutes Propidium iodide (PI) selectively binds double-stranded DNA and yields a red fluorescence PI is membrane-impermeable in healthy cells but can penetrate the membranes of late apoptotic and dead cells staining their nuclei Simultaneous staining with Annexin V-HF488 and PI enables differentiation between early apoptotic late apoptotic and dead cells
Biotang Inc Sterile PBS, 500 ml: 10 mM Sodium phosphate dibasic, 2 mM potassium phosphate monobasic, 2.7 mM potassium chloride, 137 mM sodium chloride, pH 7.4 +/- 0.2. Prepared in 18.2 megohms water. 0.22-micron filtered and/or autoclaved. BM-220S
Sterile PBS, 500 ml: 10 mM Sodium phosphate dibasic, 2 mM potassium phosphate monobasic, 2.7 mM potassium chloride, 137 mM sodium chloride, pH 7.4 +/- 0.2. Prepared in 18.2 megohms water. 0.22-micron filtered and/or autoclaved. BM-220S.
Apexbio Technology LLC Protease Inhibitor Cocktail (100X in DMSO EDTA plus) 100x(1ml A 1ml B)
This product is a protease inhibitor cocktail (100X in DMSO EDTA plus) specifically designed for use during cell lysis and protein extraction processes It is a ready-to-use concentrated solution that can inhibit a variety of endogenous proteases Also included is a separate 0 5 M EDTA solution which effectively inhibits metalloproteases The inhibitor cocktail consists of six carefully optimized broad-spectrum protease inhibitors dissolved in DMSO capable of efficiently inhibiting serine proteases cysteine proteases aspartic proteases and aminopeptidases commonly present in cell lysates EDTA is provided for inhibition of metalloproteases If immobilized metal affinity chromatography (IMAC) is involved for protein purification or if two-dimensional (2D) gel electrophoresis analysis is planned EDTA must be thoroughly removed by extensive dialysis or desalting prior to purification Specification 1 Set equals 1X (1 mL A 1 mL B) 10 Sets equals 10X (1 mL A 1 mL B) and so on
ILEX LIFE SCIENCES LLC 2-MERCAPTOETHANOL 50MM 100ML
PAN-Biotech 2-Mercaptoethanol 50 mM in DPBS (100 ml). DESCRIPTION: 2-Mercaptoethanol, also known as beta-mercaptoethanol (BME), is a potent reducing agent used in cell culture medium to prevent toxic levels of oxygen radicals. 2-Mercaptoethanol is not stable in solution, thus protocols generally require daily supplementation. PAN-Biotech 2-Mercaptoethanol is supplied at a concentration of 50 mM in Dulbecco's Phosphate Buffered Saline (DPBS). SOLUTION: 50 mM in DPBS. STORAGE: +2-8°C. STERILE: Yes. MANUFACTURER: PAN-Biotech GmbH. COUNTRY OF ORIGIN: Germany.
Medchemexpress LLC N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid | 10191-18-1 | 98.0% | 500 G
BES is a secondary standard biochemical buffer used in diagnostic testing. It offers a stable pH environment for various applications.
- Effective pH range of 6.4 to 7.8
- pKa of 7.15 at 20 °C
- Suitable for diagnostic testing areas
Boston Bioproducts Inc TAPS Buffer (0.2 M, pH 8.5) - 100ml
TAPS Buffer (0 2 M pH 8 5) - 100ml (For Research Use and Further Manufacturing Only)
Medchemexpress LLC Mops | 1132-61-2 | 99.95% | 25 G
MOPS is a buffering agent used in biology. It is known for its ability to maintain the pH of mammalian cell culture media and significantly improve the thermal stability of BSA.
- Buffering agent for biological applications.
- Maintains pH of mammalian cell culture media.
- Improves thermal stability of BSA.
eMolecules 602-25-5 | 1,4-DICHLOROANTHRAQUINONE | AstaTech | MFCD00192002 | 277.100 | C14H6Cl2O2 | 98.000 | Clc1ccc(Cl)c2C(=O)c3ccccc3C(=O)c12 | 0.25g | 475589680
1,4-DICHLOROANTHRAQUINONE | AstaTech | 602-25-5 | MFCD00192002 | 277.100 | C14H6Cl2O2 | 98.000 | Clc1ccc(Cl)c2C(=O)c3ccccc3C(=O)c12 | 0.25g | 475589680
Bioworld BES (2-(Bis(2-hydroxyethyl) amino) ethanesulfonic acid) Buffer, 0.5M, pH 6.5, 250 mL
Synonym: N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid sodium salt, BES sodium saltBelongs to the class of Good buffers, as described in the research of Dr. Norman Good et al. (1966), optimized for molecular biology and biochemistry applications.The characteristics of a Good′s buffer include: pKa between 6.0 and 8.0, high solubility, non toxic, limited effect on biochemical reactions, very low absorbance between 240-700 nm, enzymatic and hydrolytic stability, minimal changes due to temperature and concentration, limited effects due to ionic or salt composition of the solution, limited interaction with mineral cations and limited permeability of biological membranes.Technical Information :pKa (25 °C) :7.1 (Free acid)Warning: For laboratory use only.Not fit for agricultural, household, clinical use or human consumption.Appearance: LiquidPH Value: 6.5